The basic indications for immunophenotype assay in diagnosis of non-Hodgkin's lymphoma (NHL) in children including monoclonal antibodies sets are shown. Classification and description of given types of NHL in children and clinical significant of determinants expression are discussed.
The leukocytes characteristics from peripheral blood and lymph nodes including age-related changes were shown. Primary immunodeficiencies were described based on quantitative changes within lymphocytes T and B populations assayed with flow cytometry. Immunodeficiences with diagnosis based on analysis of peripheral blood and lymph node cells' immunophenotypes were discussed.