Morphological and functional disorders inserts sperm mitochondria , which are more often the cause of male infertility ( astenoteratozoospermii ) can be identified using a precise diagnosis of mitochondrial insert sperm . It includes : 1 ) a screening test for mitochondrial cytochemiczny oxidoreductase / NADH , 2 ) cytofluorometric ocenę mitochondrial membrane potential and R : 3 ) using the fluorescent mitochondrial probes , 4 ) electron - microscopically , 5) reveal abnormal biochemical respiratory chain enzymes and LDH - C4, 6 ) immunocytochemistry to detect ubiquitinated sperm mitochondria and decreased expression of keratin proteins making up the bag mitochondrial and 7 ) to detect genetic mutations in the mitochondrial genome. Many functional and morphological defects in the sperm mitochondrial sheath results from disorders of morphogenesis during its insertion spermiogenezy . These defects may be associated with apoptosis spermatids , with genetic disorders of the nuclear genome ( t haplotype ) or mitochondrial , or may occur in the epididymis . Changes in mitochondrial lower sperm motility and thus their ability to fertilize the egg . Moreover, in some cases, even the concept of obtaining (in vivo and in vitro) , may prevent the own elimination ( ubiquitination mitochondria ) in the fertilized egg cell . This can cause spontaneous abortions preimplantation , sometimes in idiopathic infertility. Highlighted the importance of cognitive diagnostics enables detection of sperm mitochondria causes astenozoospermii and forecasting to predict the chance of getting conception , especially when using assisted reproductive techniques .