FIND ARTICLE

Volume: 
Supplement: 
25
Date of issue: 

Clinical proteomics is engaged in research on the interface of chemistry , biochemistry and medicine , using modern research tools , such as , among others, microextraction and separation of complex biological samples , methods for the identification of proteins by mass spectrometry and bioinformatics . In modern proteomics includes both basic research as well as clinical research . Between these disciplines there is a continuous exchange of information ( translation) , contributing to the continued progress of knowledge about specific diseases . This process is part of systems biology , which is the understanding of how the human body functions . In fact , that in laboratory practice , the assumption is , however ambitious , faces a number of methodological difficulties . The main problem is the lack of generally accepted industry standard strategy that allows monitoring of specific diseases , and the lack of quality criteria for this , consisting of a series of steps strategy. Without the adoption of such general standards , comparing the results obtained in different laboratories is not in this situation no justification . Already at the stage of collecting biological material , there are many doubts related to the dynamics of metabolism , the influence of age, drugs , environment, how to download and storage of samples , etc. on the final result of analysis . In addition , several proteins represent more than 96 % of the total serum protein composition and concentration range between the various components dates back to 1010 . Simultaneous analysis of a huge number of data exceeds the capabilities of the standard software supplied with the measuring apparatus . The selection criteria also data obtained fundamentally affect the final result , a spectacular example of this may be one of the recent publications in which the authors analyzed the standard protein mixture was 6 , in the final stage to give a computer printout , comprising the identification of proteins of about 800 ! In light of the foregoing, it is possible at all reliable and repeatable analysis ?

Author of the article: 

The Editorial Board
Andrzej Łukaszyk - przewodniczący, Zofia Bielańska-Osuchowska, Szczepan Biliński, Mieczysław Chorąży, Aleksander Koj, Włodzimierz Korochoda, Leszek Kuźnicki, Aleksandra Stojałowska, Lech Wojtczak

Editorial address:
Katedra i Zakład Histologii i Embriologii Uniwersytetu Medycznego w Poznaniu, ul. Święcickiego 6, 60-781 Poznań, tel. +48 61 8546453, fax. +48 61 8546440, email: mnowicki@ump.edu.pl

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