Acute-phase response induced by infection, tumor growth or injury results in the release of secondary mediators - cytokines, whose role is to restore homeostasis in the body. Overproduction of cytokines can lead to many diseases, or even death. Therefore, the compounds are constantly being sought inhibitors of cytokine synthesis. The aim of this study is to characterize the action rooperolu, SB203580 and parthenolide three signal transduction inhibitors, which on one hand may be tools to study the molecular mechanisms regulating cell metabolism, on the other hand could be useful as anti-inflammatory drugs