Prostate cancer is the second most common malignancy in men worldwide. Early diagnosis of the disease makes the therapy possible however patients with metastasis have a much lower recovery chance. In vitro studies carried out worldwide in the last decade, have raised hopes of solving the problem of the molecular mechanism of metastases, but despite many experiments some aspects of the metastasis process still remain unclear. In this review we present recent information on the prostate cancer cell lines PC3, DU145 and LNCaP, derived from bone, brain, and lymph node metastases, respectively.