FIND ARTICLE

Volume: 
Supplement: 
21
Date of issue: 

An important source of vascularized organs and tissues for transplantation are heparinized donor organs ( HDN ) . The constantly growing number of downloads organ and the potential ease with which you can obtain from HDN marrow cells necessitated the need to develop effective methods for retrieving and securing them KKM , in terms of their use for transplantation . Up to now, the method of collecting bone marrow from organ donors who did not receive anticoagulant before harvesting bone marrow , were time-consuming, technically complicated and what that entails expensive. The fact that the routine administration of heparin organ donors makes the bone marrow cavities in them remains liquid . So it can obtain by simple aspiration. The subject of the research in this work were some aspects to optimize the collection and storage of cells from HDN , including the selection of a suitable anticoagulant . It should be chosen in such a way as to minimize any adverse action against hematopoietic cells . The best anticoagulant for collection and short-term storage of bone marrow was found to be a solution of heparin. Evaluation media were then used to store the living cells . It has been shown that the optimum nutrient medium to collect in the bone marrow is from RPMI HDN . It was further increased survival of hematopoietic cells , in containers open to the air , compared with those provided in a vacuum environment . The presence of pure oxygen is, however, toxic to the cells. Bone marrow cells drawn from HDN should be transported in containers containing air . In assessing the impact of mature blood cell count in the suspension on the survival of bone marrow cells stored was found that the presence of erythrocytes and granulocytes has a favorable effect on the survival of bone marrow progenitor cells by HDN during storage at 4 ° C. Described the results may in the near future to contribute to podęcia wide initiative to organize banks of human hematopoietic cells obtained from HDN . The creation of such centers would significantly expand the availability of hematopoietic cells are used for clinical purposes .

Author of the article: 

The Editorial Board
Andrzej Łukaszyk - przewodniczący, Zofia Bielańska-Osuchowska, Szczepan Biliński, Mieczysław Chorąży, Aleksander Koj, Włodzimierz Korochoda, Leszek Kuźnicki, Aleksandra Stojałowska, Lech Wojtczak

Editorial address:
Katedra i Zakład Histologii i Embriologii Uniwersytetu Medycznego w Poznaniu, ul. Święcickiego 6, 60-781 Poznań, tel. +48 61 8546453, fax. +48 61 8546440, email: mnowicki@ump.edu.pl

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