FIND ARTICLE

Volume: 
Supplement: 
25
Date of issue: 

Complete genomic sequences are a valuable source of new knowledge and open up new scientific horizons for molecular genetics and functional genomics . During the last decade, new opportunities are developing rapidly due to the development of new generation sekwenatorów WGS . With the use of these sekwenatorów it is possible to read more and longer fragments of DNA sequences while reducing the cost of reading. The result of this technological progress became the creation of new initiatives, such as Arabidopsis 1001 genomes [61 ] . In our studies aimed at determining the sequence of the genome of the cucumber used the BAC library consisting of 32 640 clones. By sequencing the ends of BAC clones were 44 276 nucleotides STC 885 , representing approximately 12.06 % of the total content of the cucumber genome coding sequences of about 23.76 %. Then, eight times the whole genome of cucumber sequenced using GS FLX Titanium sequencer , resulting in 8,000,000 fragments obtained with an average 375 nucleotides in length . This method was also used for the sequencing so . paired ends ( called paired ends) to give additional fourfold cover the whole genome sequence . At the end we made mapping and genome annotation . In this paper we present some selected and the characteristics of the genome sequence of cucumber and research activities that led to the read sequence.

Author of the article: 

The Editorial Board
Andrzej Łukaszyk - przewodniczący, Zofia Bielańska-Osuchowska, Szczepan Biliński, Mieczysław Chorąży, Aleksander Koj, Włodzimierz Korochoda, Leszek Kuźnicki, Aleksandra Stojałowska, Lech Wojtczak

Editorial address:
Katedra i Zakład Histologii i Embriologii Uniwersytetu Medycznego w Poznaniu, ul. Święcickiego 6, 60-781 Poznań, tel. +48 61 8546453, fax. +48 61 8546440, email: mnowicki@ump.edu.pl

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